{"id":3297,"date":"2024-02-16T08:39:10","date_gmt":"2024-02-16T08:39:10","guid":{"rendered":"https:\/\/www.epidemiologie.nl\/cursus\/an-introduction-to-the-analysis-of-the-next-generation-sequencing-data-el019\/"},"modified":"2024-07-04T15:41:57","modified_gmt":"2024-07-04T15:41:57","slug":"an-introduction-to-the-analysis-of-the-next-generation-sequencing-data-el019","status":"publish","type":"cursus","link":"https:\/\/www.epidemiologie.nl\/en\/cursus\/an-introduction-to-the-analysis-of-the-next-generation-sequencing-data-el019\/","title":{"rendered":"An Introduction to the Analysis of the Next-generation Sequencing Data [EL019]"},"content":{"rendered":"<p>This course provides an introduction to working with Next-Generation Sequencing (NGS) data. It targets individuals who have access to NGS data and want to learn how to work with this data and what the possibilities and limitations of NGS are. Lectures will be complemented with practical sessions in which the student will gain hands-on experience with various tools and techniques.Subjects that will be covered include:<\/p>\n<p>NGS: an introduction to methodology and techniques;<br \/>\nBasic statistics of NGS data, e.g. coverage;<br \/>\nAligning the sequence reads;<br \/>\nCalling sequence and structural variants;<br \/>\nDealing with various file formats (samtools, VCFtools, GATK);<br \/>\nAnnotating sequence and structural variants;<br \/>\nEvaluating functional effects of the genetic variants on proteins;<br \/>\nConversion to other formats;<br \/>\nSingle variant and Collapsed genotype analyses with various tools (e.g. seqMeta, RAREMETAL and RVtest);<br \/>\nFinding variants with recessive effects and compound heterozygosity;<br \/>\nSearch for rare variants in families and population based studies for complex phenotypes;<br \/>\nSearch for rare variants in Mendelian disorders, and<br \/>\nImputation of sequence variants.<\/p>\n","protected":false},"template":"","class_list":["post-3297","cursus","type-cursus","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>An Introduction to the Analysis of the Next-generation Sequencing Data [EL019] - Vereniging voor Epidemiologie<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.epidemiologie.nl\/en\/cursus\/an-introduction-to-the-analysis-of-the-next-generation-sequencing-data-el019\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"An Introduction to the Analysis of the Next-generation Sequencing Data [EL019] - Vereniging voor Epidemiologie\" \/>\n<meta property=\"og:description\" content=\"This course provides an introduction to working with Next-Generation Sequencing (NGS) data. 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